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Introducing the CytoFLEX mosaic Detection Module, where spectral flow cytometry meets modularityIntroduction to the CytoFLEX mosaic Spectral Detection Module, a modular upgrade for CytoFLEX S and LX flow cytometers that adds spectral flow cytometry capabilities. This module allows users to switch between conventional and spectral detection modes, enhancing flexibility for a wide range of experimental workflows. It is designed to improve data resolution, simplify spectral analysis, and support future-proofing of existing instruments. The module integrates with CytExpert for Spectral software and FluoroFinder’s panel design tools, offering a streamlined and user-friendly experience for designing and analyzing complex multicolor flow cytometry experiments.
Optimization of a B cell multicolor panel with CytoFLEX mosaic 88 Spectral Detection Module The document presents the development and validation of a 32-color spectral flow cytometry panel designed for detailed immunophenotyping of B cell subsets. It includes markers relevant to B cell activation, differentiation, and function, as well as additional markers for myeloid cells and chronic lymphocytic leukemia (CLL), enabling broad immune profiling. The panel was implemented on the CytoFLEX mosaic 88 spectral detection module and tested using preserved bone marrow and cryopreserved PBMCs from healthy donors. The study demonstrates that this panel is effective for extracting rich immunological data from limited sample volumes, making it a valuable tool for translational research and retrospective clinical sample analysis.