Introducing the CytoFLEX mosaic Detection Module, where spectral flow cytometry meets modularityIntroduction to the CytoFLEX mosaic Spectral Detection Module, a modular upgrade for CytoFLEX S and LX flow cytometers that adds spectral flow cytometry capabilities. This module allows users to switch between conventional and spectral detection modes, enhancing flexibility for a wide range of experimental workflows. It is designed to improve data resolution, simplify spectral analysis, and support future-proofing of existing instruments. The module integrates with CytExpert for Spectral software and FluoroFinder’s panel design tools, offering a streamlined and user-friendly experience for designing and analyzing complex multicolor flow cytometry experiments.