IOTest3 Lysing Solution is an ammonium chloride-based erythrocyte lysing solution designed for flow cytometry. This reagent is provided as a 10X-concentrated mixture of ammonium chloride, potassium bicarbonate and ethylenediamine tetraacetic acid (EDTA). IOTest3 Lysing Solution allows whole blood or bone marrow specimen preparation by erythrolysis during immunophenotyping procedures. A wash post lysis is required to yield optimal results.